BPI induces apoptosis in ECs.

(A) HUVECs were cultured for 3 days in normal culture medium; in medium with 1% human serum; or in medium containing BPI, BSA, or PF4. After partial DNA extraction and propidium iodide staining, analysis of subdiploid ECs under the different conditions was performed (n = 3, *P < .025, **P < .014). (B) Apoptosis induction by BPI is EC specific (n = 3, *P < .025). Percentage of subdiploid cells was also determined in Ls174T human colon adenocarcinoma (n = 4) and in normal human foreskin–derived fibroblasts (n = 4).