Fig. 1.
Morphologies of live and dead Burkitt lymphoma cells as determined by fluorescence microscopy.
JLP 119 cells were treated with 50 μmol/L H2O2. Cells were harvested after 22 hours, stained with Hoechst 33342 and PI, and examined by fluorescence microscopy as described in “Materials and methods.” Characteristic live and dead cell morphologies are identified by the arrows as follows: double arrow, live cell; long thin arrow, necrotic cell; long fat arrows, pyknotic/necrotic cells; short white arrow, membrane-permeable apoptotic cells; short open arrow, membrane-intact apoptotic cell.
