Decrease of Bcl-2 expression in AML cells after treatment with Bcl-2-AS.

Bcl-2 expression was determined by quantitative flow cytometry as described in “Materials and methods” after gating on live cells. Results are represented as mean ± SEM of the ABC of sensitive (responsive) and resistant (nonresponsive) cells at baseline (preculture) and after treatment with Bcl-2-AS, NS, and in control cultures without ODNs. In both types of cells, 8 μmol/L of Bcl-2-AS caused a significant (P < .05) decrease in Bcl-2 expression.