Fig. 4.
EPO prevention of the TRAIL-mediated depletion of glycophorin Ainterm erythroblasts.
The expression of glycophorin A was evaluated on viable cells electronically gated on the basis of FSC/SSC pattern (see caption of Figure 1) at day 10 of culture with the indicated cytokines. Cultures were supplemented (bottom 2 panels) or not with fresh EPO for 12 hours and then with TRAIL or rHis6-tag peptide (CONT) for the last 20 hours of culture before glycophorin A staining. X-axis: glycophorin A relative expression. Y-axis: relative cell number. Percentages of glycophorin A+ cells are indicated.
