Dominant-negative form of Rac1 and CDC42 inhibits the B6H12-induced transmigration on FN without affecting the avidity/affinity of 4β1 (VLA-4).

The polarization assay on the immobilized FN (A) and the transmigration assay to FN (B) were performed in the presence of the indicated mAb (10 μg/mL) in each stable clone, as described in “Materials and methods.” The adhesion assay using crystal violet method (C) and the FN binding assay (D) in the presence of the indicated mAb in each stable clone were performed, as described in “Materials and methods.” Data are mean ± SD of 3 independent experiments. Statistically significant differences from control values are indicated by *P < .01 and **P < .05. Solid bars, vector. Open bars, N17Rac1N6. Hatched bars, N17CDC42N102.