![Fig. 5. Anti-IAP/CD47 mAb (B6H12) promotes the migration to FN through 4β1 (VLA-4) in BALL cells. / (A) After the preincubation of B6H12 (10 μg/mL) at 4°C for 30 minutes, the BALL cells (5 × 104) were incubated on the 96-microtiter wells coated with FN (10 μg/mL) in the presence of soluble B6H12. After 2 hours of incubation, the rate of polarized cells was counted. (B) Polarization assay was performed in the presence or absence of anti-β1 mAb (SG19), as described in A. Data are shown mean ± SD of 3 independent experiments. (C) After preincubation of anti-IAP/CD47 mAbs as described above, the cells (1 × 105) were applied into the cell culture insert prepared as described in “Materials and methods.” After 24 hours of incubation, cells that transmigrated to the lower chamber were counted. (2D3, B6H12; anti-IAP/CD47, MOPC195; mouse IgG2b). Representative data are shown from 3 independent experiments. (D) The transmigration assay using Boyden chamber was performed in the presence or absence of anti-β1 mAb (SG19), as described in C. Data are shown mean ± SD of 3 independent experiments. Statistically significant differences from control values are indicated by *P < .01. Percentage inhibition was calculated as the following formula: % inhibition = [(% transmigration with B6H12 − % transmigration with B6H12 and SG19)/(% transmigration with B6H12 − % transmigration with MOPC21)] × 100.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/1/10.1182_blood.v96.1.234/4/bloo01306005x.jpeg?Expires=1722773811&Signature=2DA8rReuEyvqj3xTX6LzOeuKNTCeer8vUZMo~ShIG7UPRPAILmFmjy6ysVOtL7aucWdzP3BhcR3~2WZ0qqylxl~3lx77S0IhzmkKu23m4k2qrdB5e7YYwtvZBSKDuMBIYvV~13DQx4HlDSy52lZ35Eerq4i2W5RUTFRboia1u3dvRZerG8XAS3PJodM1Z1wkIjm~zDa8BWMrc-HiHSE51RG89XbSPDw1eXRzPWUyiPvvdpVAduQpDdbcKxDSiJfvDWk63qjr-gbxNgMzaTZpVjiiDR1blBwgYJWFU15pE~rWKd2XNmVUwqzXap7VURKvj~qpyTUbmpDtMZWpx~gN3A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Anti-IAP/CD47 mAb (B6H12) promotes the migration to FN through 4β1 (VLA-4) in BALL cells.
(A) After the preincubation of B6H12 (10 μg/mL) at 4°C for 30 minutes, the BALL cells (5 × 104) were incubated on the 96-microtiter wells coated with FN (10 μg/mL) in the presence of soluble B6H12. After 2 hours of incubation, the rate of polarized cells was counted. (B) Polarization assay was performed in the presence or absence of anti-β1 mAb (SG19), as described in A. Data are shown mean ± SD of 3 independent experiments. (C) After preincubation of anti-IAP/CD47 mAbs as described above, the cells (1 × 105) were applied into the cell culture insert prepared as described in “Materials and methods.” After 24 hours of incubation, cells that transmigrated to the lower chamber were counted. (2D3, B6H12; anti-IAP/CD47, MOPC195; mouse IgG2b). Representative data are shown from 3 independent experiments. (D) The transmigration assay using Boyden chamber was performed in the presence or absence of anti-β1 mAb (SG19), as described in C. Data are shown mean ± SD of 3 independent experiments. Statistically significant differences from control values are indicated by *P < .01. Percentage inhibition was calculated as the following formula: % inhibition = [(% transmigration with B6H12 − % transmigration with B6H12 and SG19)/(% transmigration with B6H12 − % transmigration with MOPC21)] × 100.
