Dominant-negative forms of Rac1 and CDC42 significantly suppress the B6H12-induced polarization in BALL cells.

(A) The stable clones expressing either N17Rac1 (N2, N4, and N6) or N17Cdc42 (N2, N9, and N102) were obtained as described in “Materials and methods.” After the incubation on the immobilized B6H12 (10 μg/mL) for 5 hours, the rate of polarization of each clone was calculated. Data are shown as mean ± SD in 3 independent experiments. Data of immunoblotting using anti-myc mAb 9E10 in each whole-cell lysate are shown in the lower panel. (B) Time-course analysis of the B6H12-induced polarization of N17Rac1- and N17CDC42- transfected BALL cells. Representative data are shown as mean ± SD in 3 independent experiments. Two other independent experiments showed similar results. Open circle, parent. Closed circle, vector. Square, N17Rac1N6. Triangle, N17CDC42. Other clones showed similar results.