Fig. 2.
Fig. 2. Phase-contrast microscopy and immunofluorescence staining of BALL cells that express either N17Rac1 or N17CDC42. / Cells were cultured on the culture slide coated with either MOPC195 or B6H12 (10 μg/mL) for 5 hours. The morphology of the BALL cells was photographed under phase-contrast microscopy. For immunofluorescence staining, the cells were fixed and stained with either anti-mycmAb 9E10 or phalloidin, as described in “Materials and methods.” (A-C) Phase-contrast micrograph in the incubation on immobilized B6H12. (D-F) Immunofluorescence staining with phalloidin on immobilized B6H12. (G-I) Immunofluorescence staining with 9E10 on B6H12. (A, D, G) Vector. (B, E, H) N17Rac1N6. (C, F, I) N17CDC42N102. Initial magnifications, × 400 in A-C and × 1000 in D-I.

Phase-contrast microscopy and immunofluorescence staining of BALL cells that express either N17Rac1 or N17CDC42.

Cells were cultured on the culture slide coated with either MOPC195 or B6H12 (10 μg/mL) for 5 hours. The morphology of the BALL cells was photographed under phase-contrast microscopy. For immunofluorescence staining, the cells were fixed and stained with either anti-mycmAb 9E10 or phalloidin, as described in “Materials and methods.” (A-C) Phase-contrast micrograph in the incubation on immobilized B6H12. (D-F) Immunofluorescence staining with phalloidin on immobilized B6H12. (G-I) Immunofluorescence staining with 9E10 on B6H12. (A, D, G) Vector. (B, E, H) N17Rac1N6. (C, F, I) N17CDC42N102. Initial magnifications, × 400 in A-C and × 1000 in D-I.

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