Fig. 1.
Fig. 1. Immobilized anti-IAP/CD47 mAb (B6H12) induces the polarization in human B cell lines. / (A) Phase-contrast micrographs of BALL cells on immobilized anti-IAP/CD47 mAbs. BALL cells were cultured on the plates coated with each mAb (10 μg/mL) for 5 hours. Immobilized B6H12 could induce the elongated and head–tail morphology (polarization) in BALL cells. (B6H12, 2D3: anti-IAP/CD47 mAb; MOPC195: mouse IgG2b). Initial magnification, × 400. (B) The rate of polarization induced by the immobilized B6H12 in human B-cell lines. Cells were cultured on the plates immobilized with B6H12, and the rate of polarization was calculated as described in “Materials and Methods.” In 2 pre-B cell lines (BALL and Nalm6) and 2 lymphoma cell lines (ONHL-1 and Daudi), the polarization on the immobilized B6H12 was prominently observed. Data are shown as the mean ± SD in 3 independent experiments.

Immobilized anti-IAP/CD47 mAb (B6H12) induces the polarization in human B cell lines.

(A) Phase-contrast micrographs of BALL cells on immobilized anti-IAP/CD47 mAbs. BALL cells were cultured on the plates coated with each mAb (10 μg/mL) for 5 hours. Immobilized B6H12 could induce the elongated and head–tail morphology (polarization) in BALL cells. (B6H12, 2D3: anti-IAP/CD47 mAb; MOPC195: mouse IgG2b). Initial magnification, × 400. (B) The rate of polarization induced by the immobilized B6H12 in human B-cell lines. Cells were cultured on the plates immobilized with B6H12, and the rate of polarization was calculated as described in “Materials and Methods.” In 2 pre-B cell lines (BALL and Nalm6) and 2 lymphoma cell lines (ONHL-1 and Daudi), the polarization on the immobilized B6H12 was prominently observed. Data are shown as the mean ± SD in 3 independent experiments.

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