Synergistic activity of CIITA with RelA and IRF1.

(A) MOLT4 cells were transfected with the HLA-B CAT reporter along with 0.1 or 0.5 μg of expression vectors for CIITA, RelA, or IRF1. The activity with all 3 expression vectors was defined as 100 U. (B) RelA (0.5 μg) and CIITA (0.1 μg) expression vectors were cotransfected with reporter plasmids consisting of the HLA-BENH-A/IRE region placed upstream of the basal metallothionein promoter24 (left panel) or the DRA promoter (right panel).