Anti-MCP-1 inhibited lung tumor metastasis in mice bearing MDA-231 tumors.
SCID mice were injected intravenously with 20 μL of antiserum to ASGM1, on day 0, and 3 × 105 MDA231 human breast carcinoma cells were injected intravenously on day 1. Antibodies, including rabbit IgG and anti-MCP-1 at 25 μg/mouse (1 mg/kg), were given intraperitoneally to the mice on days 4, 8, 12, 16, 20, 24, and 28. For experimental metastasis experiments, mice from both groups were sacrificed on the 35th day after intravenous injection of the tumor cells. Lungs were extracted and fixed in formalin. Histological sections were stained with hematoxylin and eosin. By using the Bioquant Program, the area of the total tissue per field 40× field (D1) was determined. The micrometastases present within the same field were gated, and the area within the gates was counted (D2). The metastatic index was calculated by the ratio D2/D1. A minimum of 20 fields was analyzed per slide in a blinded manner, and 8 mice were used per group in each experiment. The experiment was repeated 2 times. The figure shows the data obtained from the 2 separate experiments. The mean and SEM for each group are indicated. Statistical analysis between the 2 groups was performed using Student t test (P < .005).
