Changes in renal vascular permeability in rats after renal I/R, and effects of APC, DEGR-FXa, heparin, DIP-APC, and leukocytopenia on the I/R-induced increase in vascular permeability observed 6 hours after reperfusion.

(A) Rats were subjected to uninephrectomy and 60 minutes of renal ischemia followed by reperfusion. Renal vascular permeability was evaluated by assessing leakage of Evans blue dye administered intravenously 10 minutes before the rats were killed. Vascular permeability was measured before ischemia (Pre) and 3, 6, 12, and 24 hours after reperfusion. Data are expressed as mean ± SD values. Open circles indicate rats in the sham-operation group (n = 5), and closed circles, rats that had I/R (n = 5). *P < .01 compared with the sham-operation group. (B) Rats were given intravenously administered saline, APC (100 μg/kg), DEGR-FXa (1 mg/kg), heparin (300 U/kg), or DIP-APC (100 μg/kg) 30 minutes before reperfusion. Leukocytopenia was induced by administration of nitrogen mustard. Data are expressed as mean ± SD values. Values in parentheses indicate the number of animals in each experiment. *P < .01 compared with the sham-operation group; †P < .01 compared with the I/R plus saline group.