KF41399 administration increased the expression level of Bcl-2 protein in Lin⁻ cells.

Mice were treated with 25 mg/kg KF41399 orally twice daily for 3 days (protocol B, Table 1). Control animals were administered distilled water instead of KF41399. Lin⁻ cells were purified on day 3, and cell lysate was prepared. The expression level of each protein was detected, as described in “Materials and methods.” Bcl-2 (A), bax (B), and β-actin (C).