KF41399 administration modulated the expression level of cell cycle regulating proteins in Lin⁻ cells.

Mice were treated with 25 mg/kg KF41399 orally twice daily for 3 days (protocol B, Table 1). Control animals were administered distilled water instead of KF41399. Lin⁻ cells were purified on day 3, and cell lysate was prepared. The expression level of each protein was detected as described in “Materials and methods.” Cyclin D1 (A), cyclin E (B), cyclin A (C), Cdk4 (D), Cdk6 (E), Cdk2 (F), Rb (G), p130 (H), and β-actin (I). Phosphorylation status of Rb was shown as ppRb (hyper-phosphorylated Rb), pRb (hypo-phosphorylated Rb), and Rb (unphosphorylated Rb).