Oral administration of KF41399 reduced the toxicity caused by ACNU.

In the ACNU-treated group, mice were administered 45 mg/kg ACNU IV for 2 consecutive days on day 3 and day 4 (protocol I, Table 1). In the KF41399 pretreated group, mice were treated with 25 mg/kg orally KF41399 twice daily for 3 days, from day 0 to day 2. This was followed by the IV administration of 45 mg/kg ACNU for 2 consecutive days on day 3 and day 4 (protocol D, Table 1). Control animals were administered distilled water (protocol A, Table 1). Body weight on day 14 (A), spleen weight on day 14 (B), and number of bone marrow-derived MNC per femur and tibia on day 7 (C) were measured as described in “Materials and methods.” Results are expressed as the mean ± SD. n = 5 in each group.