Persistence of F+ cell proportions during the later culture phase.

Cultures were incubated with TGFβ or FCS for 4 days, then washed and reseeded in fresh control medium. On days 7 and 10, the cultures were further diluted (1:10 and 1:5, respectively) in fresh control medium (full symbols) or medium supplemented with TGFβ1 (open symbols). Percentage of F+ cells was determined at selected times between day 7 and day 13. The time course is shown from 1 individual experiment in which all conditions were investigated together on the cells from the same donor. The vertical bars on days 7, 10, and 13 show the ranges of results obtained in 4 independent experiments with blood from 4 different donors.