RHD gene deletion in the Rh-negative haplotypes.

Three of the 3100-bp segments of the Rhesus boxes are shown. The upper line indicates the nucleotide sequence of the upstreamRhesus box in D-positive haplotypes; the lower line indicates the nucleotide sequence of the downstream Rhesus box in D-positive haplotypes. The middle line gives the nucleotide sequence of the single Rhesus box carried by Rh-negative haplotypes. The asterisk symbols denote identical nucleotides. TheRHD deletion occurred in a 903-bp segment of absolute identity that was part of a 1463-bp identity region. The positions of primers rez7 and rnb31 are shown (m indicates mismatch).PstI restriction sites are indicated by upward carets. The 3 Rhesus boxes were deposited at EMBL under accession numbers AJ252311 (upstream Rhesus box), AJ252312(downstream Rhesus box), and AJ252313 (hybrid Rhesus box).