Multimer analysis of rvWF-WT, type 2M and type 2B rvWFs.

rvWF multimers were resolved by 0.1% SDS/1% agarose gel electrophoresis and detected by ¹²⁵I-MoAb anti-vWF. rvWF-WT, type 2M rvWF (-I662F, -G561A, -E596K) and type 2B rvWF-L697V exhibited a normal multimeric pattern, whereas rvWF-R611H displayed a normal multimeric composition but a decreased intensity of the highest molecular weight multimers. NP indicates normal plasma.