Effect of U0126 on the production of IL-1β induced by β2 integrin engagement.
Panels A-D: Effects of U0126 on the level of pro–IL-1β mRNA induced by ligation of β2 integrins. Left-hand side: Monocytes were preincubated for 30 minutes with increasing concentrations of U0126 and then stimulated with anti-CD11b (5 μg/mL, A), anti-CD11c (5 μg/mL, B), MBP-CD23 (1 μg/mL, C) or ZZ-CD23 (1 μg/mL, D) for 1 hour at 37°C. Right-hand side: Cells were untreated or preincubated for 30 minutes with U0126 (40 μmol/L) and then stimulated for the indicated times with anti-CD11b (5 μg/mL, A), anti-CD11c (5 μg/mL, B), MBP-CD23 (1 μg/mL, C) or ZZ-CD23 (1 μg/mL, D). Panel E: Dose-dependent inhibitory effect of U0126 on the production of IL-1β. Human monocytes (150 × 103 cells per well) were pretreated for 30 minutes with various concentrations of U0126 and then incubated for an additional 16 hours with anti-CD11b (20 μg/mL), anti-CD11c (5 μg/mL), ZZ-CD23 (2 μg/mL), or MBP-CD23 (1 μg/mL). IL-1β production was determined in total cell lysates by ELISA. Data are means from triplicates of 1 experiment representative of 2 others (in each condition the SD was less than 5%).
