Determination of the transcriptional activity ofpro–IL-1β gene in isolated nuclei from human monocytes stimulated by β2 integrin engagement.

Nonadherent human monocytes (30 × 10⁶ cells) were starved overnight in RPMI 1640 medium supplemented with 1% FCS and then stimulated for 1 hour with anti-CD11a (2 μg/mL), anti-CD11b (2 μg/mL), anti-CD11c (2 μg/mL), MBP-CD23 (1 μg/mL), ZZ-Eselectin (1 μg/mL), or ZZ-CD23 (1 μg/mL). Then cells were lysed, nuclei isolated, and in vitro transcription performed as described in “Materials and methods.” Results are representative of 3 distinct experiments.