Induction of IL-1β production by engagement of β2 integrins on the surface of freshly isolated human monocytes.
Human monocytes (150 × 103 cells per well) were incubated for 16 hours with antibodies or recombinant fusion proteins at the following concentrations: isotype control IgG1 (5 μg/mL); anti-CD11a (clone BU17, 5 μg/mL); anti-CD11b (clone ICRF44, 20 μg/mL); anti-CD11c (clone BU15, 5 μg/mL); ZZ-CD23 and MBP-CD23 at 0.5 μg/mL; and ZZ-Eselectin and ZZ-Pselectin at 5 μg/mL. Secreted IL-1β was detected by ELISA in the culture supernatants, total production of the cytokine being determined in total cell lysates after addition of 0.1 volume of 10% NP40 to monocytes. Data are mean ± SD from triplicates of 1 experiment representative of 4 others. Similar results were obtained with anti-CD11a (clone G43-25B), anti-CD11b (clone 44), and anti-CD11c (clone 3.9) mAbs (not shown).
