Effect of PD98059 on ERK phosphorylation in v-fes–overexpressing macrophages.

VFB cells were incubated for 16 to 18 hours in DMEM supplemented with 10% FBS and treated or not for 30 minutes or overnight (o/n) with 30-μmol/L PD98059. Equal amounts of cell lysates were separated by SDS-PAGE in a 12.5% gel and immunoblotted with polyclonal α-pERK antibody (A) or, after stripping, α-ERK antibody (B).