TOR enhances CD86 expression by mechanisms not involving the ER.

Adherent PBMC were cultured with IL-4 + GM-CSF in the presence or absence of TOR, ICI 182780, and/or E2 in a culture medium containing dextran-charcoal stripped FCS without phenol red (see “Materials and methods”). After 48 hours the cells were stained for CD86 expression and analyzed with flow cytometry. The data represent MFIRs (see “Materials and methods”) of 3 individual experiments (mean ± SD).