Effect of PD98059 on the growth of v-fes–overexpressing macrophages.

VFB cells were plated in 96-well culture plates at 10 000 cells/well, and the accuracy of plating was verified 3 hours later (t = 0 line). Cells were incubated in DMEM supplemented with 10% FBS in the absence (white columns) or the presence (gray columns) of 30-μmol/L PD98059 and counted 3 days later. Data are means ± SEM of triplicate samples of a typical experiment.