Monocytes, immature DC, and mature DC express mRNA for ER- and ER-β.

CD14⁺, CD1a⁺, and CD83⁺ cells were sorted with MACS as described in “Materials and methods.” The hybridized PCR products of ER-α and ER-β are shown. MCF-7 breast cancer cells were used as a positive control for the ER mRNA expression. RT was omitted from the control reactions. The constitutively expressed β-actin is shown in the bottom panel.