Fig. 3.
![Fig. 3. TOR and IL-10 inhibit the function of monocyte-derived DC. / Adherent PBMC were cultured in a medium alone (control) or with IL-4 + GM-CSF in the presence or absence of TOR or IL-10 (100 U/mL). After 7 days of culture, the cells were washed, irradiated (30 Gy), and counted. Five to 20 × 103 cells were then cocultured with 2 × 105 allogeneic PBMC for 5 days, after which the proliferative activity was measured. The results are expressed as mean counts per minute ([cpm] ± SD) of a representative experiment made in triplicate. Similar results were obtained in another 2 experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/9/10.1182_blood.v95.9.2875.009k12_2875_2882/5/bloo00912003x.jpeg?Expires=1724268272&Signature=vN4P6EV8cIv0Mz27ef3QGqestgr0p3jyZUDC4vm~hOlwkwqYEdwFb2CL~Yggl4MGLW26nd00LuSfj8khD7ox9eXGZSLkHjGWxIc5QzJ1SccdF~IzDENjOeQrICwyTobX965EmBoeu4PrTQIq0lpMyrJAli3zSc9-UtMwmPKDvCf-IsUps9JT5A~6cMhg51TWZ9QpZYS8ELYzH8uHjL5bPD1I~foU~k3qik9F0NJOwnW7Y3QNflsqsPx4~mMcrRk79yPFsdb0EoYZZzPUPuNqwNfTw5gGm2K4C0IeD66txW3SDYfGDafesi3kAxK2iXmXCx36TyNikS3PskCWnNGiWA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TOR and IL-10 inhibit the function of monocyte-derived DC.
Adherent PBMC were cultured in a medium alone (control) or with IL-4 + GM-CSF in the presence or absence of TOR or IL-10 (100 U/mL). After 7 days of culture, the cells were washed, irradiated (30 Gy), and counted. Five to 20 × 103 cells were then cocultured with 2 × 105 allogeneic PBMC for 5 days, after which the proliferative activity was measured. The results are expressed as mean counts per minute ([cpm] ± SD) of a representative experiment made in triplicate. Similar results were obtained in another 2 experiments.
