Analysis of CD34 expression of purified subpopulations after in vitro culture for 3 days. A representative experiment (n = 5) of CD34 cell surface expression performed on subpopulations of cells isolated from the CD34⁻CD38⁻Lin⁻population based on the absence or presence of (A) CD7 or (B) AC133 cell surface expression. The entire contents of individual wells was collected after 3 days (2000-10 000 cells), washed, stained with mAbs against CD34, and analyzed using flow cytometric analysis. Cell surface phenotypes of subpopulations prior to culture are indicated as Day 0 (A, B) within isotype control dot plots.