American Society of Hematology

Fig. 1.

$Fig. 1. Phenotypic analysis of cell surface AC133 and CD7 on primitive subpopulations of CD34−CD38−Lin− and CD34+CD38−Lin−populations of human CB cells. Representative analysis of cell surface CD34 and CD38 by multiparameter flow cytometry of human CB cells depleted for cells expressing lineage commitment markers (Lin−). (A) Subpopulations of CD34−CD38−Lin−and CD34+CD38−Lin−cells were gated R1 and R2, respectively. Histograms showing the expression of CD7 and AC133 on gated subfractions of (B, C) CD34−CD38−Lin−cells and (D, E) CD34+CD38−Lin− cells. Due to the low number of AC133 cells detected in the CD34−CD38−Lin−fraction, cells shown in (C) are also gated CD7−using (B) markers. (A to E) Markers indicate sorting gates used for the isolation of specified subfractions. Analysis is representative of 4 independent CB samples. A single-line histogram indicates isotype overlay.$

Phenotypic analysis of cell surface AC133 and CD7 on primitive subpopulations of CD34⁻CD38⁻Lin⁻ and CD34⁺CD38⁻Lin⁻populations of human CB cells. Representative analysis of cell surface CD34 and CD38 by multiparameter flow cytometry of human CB cells depleted for cells expressing lineage commitment markers (Lin⁻). (A) Subpopulations of CD34⁻CD38⁻Lin⁻and CD34⁺CD38⁻Lin⁻cells were gated R1 and R2, respectively. Histograms showing the expression of CD7 and AC133 on gated subfractions of (B, C) CD34⁻CD38⁻Lin⁻cells and (D, E) CD34⁺CD38⁻Lin⁻ cells. Due to the low number of AC133 cells detected in the CD34⁻CD38⁻Lin⁻fraction, cells shown in (C) are also gated CD7⁻using (B) markers. (A to E) Markers indicate sorting gates used for the isolation of specified subfractions. Analysis is representative of 4 independent CB samples. A single-line histogram indicates isotype overlay.

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