Promoter activity of the 5′ flanking region of the mouse RAG-2 in lymphoid cells.

Schematic diagram of luciferase reporter constructs fused to RAG-2 promoter sequences is shown on the left, and the promoter activity in various lymphoid cells is on the right. For the luciferase reporter constructs and the pSRα-LacZ reference plasmids, 5 μg of each were transfected into 5 × 10⁶ mouse lymphoid cell lines (see “Materials and methods”), and their activities were assessed 22-24 hours later. Luciferase activity was normalized with β-galactosidase activity, and luciferase construct without a promoter fragment was given a reference value of 1. Error bars indicate deviation of 3-4 experiments.