Expression of REDK in CD34⁺ cells during erythroid differentiation.

Purified CD34⁺ cells were seeded in medium (20% FBS) containing 4 U/mL EPO and 50 ng/mL SCF. Cells were harvested for RNA extraction at the days indicated, and RT-PCR analysis was performed with REDK-L– and REDK-S–specific primers. Quantitation of the relative expression of the REDK-L and REDK-S messages was determined based on normalization to G3PDH.