p53 stabilization and transcriptional impairment in ex vivo ATLL cells.
(A) Top panel: Western blot of total cellular lysates from ATLL cells from patients 1 to 4 and 6 or from HTLV-I carrier (HC 5) or normal human PBMC. Bottom panel: Northern blot analysis of the GADD45 and p21WAF1 expression after ionization–radiation treatment (+) of ATLL cells from patients 1, 2, and 4 and from HC 5.5In patients 7 to 10, protein lysates for p53 analyses were unavailable. The numbers designate the same patients in both top and bottom panels of the figure. (B, C) Quantitative summaries of the data presented in the bottom part of A as fold induction of GADD45 mRNA (B) or p21WAF1 mRNA after ionization–radiation. Results were obtained from a single experiment in each patient because of the intrinsic limitation of cells available.
