Cell death analysis of FL5.12 cells transiently transfected with pIRES-EGFP-Hrk.

(A) Schematic structure of the bicistronic vector containing the internal ribosomal entry site (IRES) and the Hrk and EGFP cDNAs. (B) Flow cytometry analysis of cells after 24 hours of transfection with pIRES-EGFP containing Hrk in the sense or inverted orientation (io). Quadrants in the dot plots were set according to the green fluorescence of cells transfected with a negative control vector (pIRES-Neo). Histograms represent the percentage of cells stained with propidium iodide in the green fluorescence positive population (R1) and the green fluorescence negative population (R2). All histograms and dot plots are from a representative experiment (n = 3).