Analysis of Hrk and apoptosis in FDCP-Mix cells treated with ionomycin.

Cells were cultured for 24 hours with ionomycin in the presence of IL-3 and then analyzed. As controls, cells cultured in the presence or absence of IL-3 were also analyzed. (A) Expression of Hrk and Bax by Western blot. (B) DNA fragmentation analysis by electrophoresis onto a 2% agarose gel and staining with ethidium bromide.