Fig. 3.
Fig. 3. Expression of SMAD5β in hematopoietic and leukemic cells. / A representative autoradiograph of multiplexed RT-PCR products resolved on a poly-A site. The upper band (307 bp) representsSMAD5β; the middle band (170 bp) represents the full-length SMAD5; and the lower band (114 bp) represents the internal control, β-2-m. Templates used for amplification are as indicated: peripheral blood mononuclear cells (PBMCs), normal bone marrow progenitor cells (CD34+), acute myelogenous leukemia (ML-3), erythroleukemia (TF1), acute myelogenous leukemia (AML193), erythroleukemia (HEL), acute myelogenous leukemia (KG1), chronic myelogenous leukemia in erythroid blast crisis (K562), and myelomonocytic leukemia (U937).

Expression of SMAD5β in hematopoietic and leukemic cells.

A representative autoradiograph of multiplexed RT-PCR products resolved on a poly-A site. The upper band (307 bp) representsSMAD5β; the middle band (170 bp) represents the full-length SMAD5; and the lower band (114 bp) represents the internal control, β-2-m. Templates used for amplification are as indicated: peripheral blood mononuclear cells (PBMCs), normal bone marrow progenitor cells (CD34+), acute myelogenous leukemia (ML-3), erythroleukemia (TF1), acute myelogenous leukemia (AML193), erythroleukemia (HEL), acute myelogenous leukemia (KG1), chronic myelogenous leukemia in erythroid blast crisis (K562), and myelomonocytic leukemia (U937).

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