Recombinant MIP-1 inhibits HIV-1 replication in CD8-depleted PBMC cultures of monoinfected individuals.

PBMCs were isolated from 3 individuals with progressive HIV-1 disease, and CD8⁺ T cells were removed by a single round of immunomagnetic beads (Dynal, Oslo, Norway). rMIP-1α (100 ng/mL) that was added twice (time 0 and 72 hours from the establishment of the culture) inhibited virus replication in these autologous cell cultures, although with variable potency.