Summaries of the c-abl Pa promoter and p15 5′ CpG island methylation levels.

(A) Summary of the c-abl Pa promoter methylation levels of 1 patient or healthy control. Each vertical bar represents the average of 2 CpG sites by Ms-SnuPE assay. CD34⁺ indicates a CD34⁺ enriched sample from the peripheral blood of a hematologically normal patient; WBC, white blood cell sample from a healthy individual; CH, chronic phase of CML; AC, accelerated phase of CML; BC, blast crisis phase of CML; ALL, acute lymphocytic leukemia; AML, acute myelogenous leukemia; and MDS, myelodysplastic syndrome. (B)(a) Summary of the p15 5′ CpG island methylation levels in CMLs and controls by Ms-SnuPE assay. The levels shown represent the average of 3 CpG sites. BM indicates normal bone marrow; WBC1 and WBC2, blood DNAs from healthy donors; CH, chronic CML; AC, accelerated CML; and BC, blast crisis CML. (b) Southern blotting also showed methylated p15 5′ region status in the CML patients. The following are depicted: lane 1, undigested WBC DNA; lane 2, EagI digested WBC DNA from the healthy control; lane 3, CML accelerated samples; and lane 4, CML blast samples. The samples in lanes 3 and 4 are methylated because a larger band appeared after digestion with EagI, similar to the uncut DNA in lane 1.