Ms-SNuPE standard curve for the p15 5′ untranslated region (UTR) showing the quantitative results obtained from the in vitro SssI methylated DNA standards.

WBCs, which are unmethylated, are from the peripheral blood of a healthy donor, while the Raji is a leukemic cell line that is a positive methylated control. Methylation is a measure of the signal in the C lane, which represents the amount of methylated DNA molecules as a result of the [³²P]dCTP incorporation. A band in the T lane is the product of [³²P]dTTP incorporation and represents unmethylated cytosine molecules at a particular CpG site. NDC indicates no DNA control; 1, 2, and 3 are individual cytosines in a CpG site examined by each Ms-SNuPE primer; and T is the control for a complete bisulfite conversion, representing a C not in a CpG dinucleotide.