Interleukin-6 (IL-6) transcripts in bone marrow stromal cells (BMSCs) and microvascular endothelial cells (MVECs) after exposure to vascular endothelial growth factor (VEGF).

Adherent cells were incubated with 50 ng/mL of VEGF165 or VEGF121 for 6 and 24 hours, respectively, and analyzed for IL-6 expression by RT-PCR. Levels of IL-6 messenger RNA (628 base pairs) were estimated by normalization against co-amplified glyceraldehyde-3-phosphate dehydrogenase. C = unstimulated controls; M = size marker.