Fig. 5.
![Fig. 5. Specificity of lipid binding to EDG-6 . HEK293 cells were transfected with EDG-6 and binding of 0.5 nmol/L [32P]SPP was determined in the presence of the indicated concentrations of unlabeled lipid competitors. Results are means ± SD of triplicate determinations. Competition curves were fit and Kis calculated using Deltagraph program for Macintosh. Below, shown are the Ki values and correlation coefficients (r2) of the curve fits for SPP, dihydro-SPP, and SPC. LPA did not compete at any concentration tested.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/8/10.1182_blood.v95.8.2624/4/bloo00833005x.jpeg?Expires=1724940421&Signature=KBMcwjnij~jBmLb-u5kKcq5pqNBuHZ2yZ6058rpD5BczV~h7DqhN~si1bqdaUzTLNsG9t-ge6ECzbZ6040ff9sStGR1l4Xff1~67mG5ONs0iS~G3VXYu42-9wqYg-vmo3O2ihyNgav1FrkzMwV6D5m5hMT76Mei7GnRLOBPsw4-GGIgzCqu5U7TyMWJFwcqjVZGJBzhtlBYX5e~thng9f2RZSGT0BfmwDc2d2UjNcmfP06B1FTLWJ~qDaZTa7o5JEa1W-YZP7riyanVOZ2w1pyMNNhhtPRrPb3npQX3QsnDCxqyt3NkPXsVRbl7HASeRi7b64QCtVWqyiZ1iTrZ1kw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Specificity of lipid binding to EDG-6 . HEK293 cells were transfected with EDG-6 and binding of 0.5 nmol/L [32P]SPP was determined in the presence of the indicated concentrations of unlabeled lipid competitors. Results are means ± SD of triplicate determinations. Competition curves were fit and Kis calculated using Deltagraph program for Macintosh. Below, shown are the Ki values and correlation coefficients (r2) of the curve fits for SPP, dihydro-SPP, and SPC. LPA did not compete at any concentration tested.
