Generating and plotting individual standard curves.

(A) Mixing experiments for patient UPN 251 by PCR with D1S80 primers for generating an individual standard curve. Recipient (R) DNA was mixed with donor (D) DNA in various proportions as indicated. (B) Evaluating locus D1S80 of patient UPN 251 for generating the standard logarithmic curve. After amplification and densitometric analysis of the chimeric donor and recipient samples, a standard curve was generated by plotting the percent recipient DNA versus the mean values of quotient recipient/donor DNA.