Characteristics of malignant ascites and resultant SKI-DLCL-1 cell line.

(A) Cells were fixed and stained with May–Grünwald–Giemsa (magnification, ×100). SKI-DLCL-1 cells and cells from the original ascitic fluid demonstrate identical cytologic features consistent with a diagnosis of large-cell lymphoma. (B) Flow cytometric analysis of the ascites and the SKI-DLCL-1 with a panel of monoclonal antibodies. (C) Cytogenetic analysis of the diagnostic ascites and the resultant cell line demonstrates similar though nonidentical findings. This likely represents clonal selection by the process of establishing the cell line.