Expression of PRV-1 in peripheral blood granulocytes from patients with PV and normal controls.

Granulocytes were isolated from peripheral blood to more than 98% purity. Total RNA was extracted and a Northern blot prepared using 10 μg RNA. Lanes 1-5 healthy volunteer donors, lanes 6-10 patients with PV. (Top) The membrane was probed with a 1.1-kb fragment of the PRV-1 cDNA. The positions of the 18S and 28S ribosomal RNAs are indicated. (Bottom) The membrane was stripped and reprobed with a 1.2-kb fragment of the human β-actin cDNA.