Tyrosine phosphorylation of GTPases in neutrophils from healthy individuals and SCN patients.

GTPases were immunoprecipitated from neutrophil cytosol (500 μg protein each), separated on a 8% SDS-PAGE, blotted onto nitrocellulose membranes, and detected with (A) anti-PY antibody, or (B) anti-RhoA or anti-Rac2 antibodies. These results were representative for 3 independent experiments with cells from 3 SCN patients and 3 healthy individuals. N indicates normal neutrophils, and SCN, patients' neutrophils.