Association of GTPases and GDIs in neutrophils from healthy individuals and SCN patients.

GDIs were immunoprecipitated from neutrophil cytosol (500 μg protein each), separated on a 8% SDS-PAGE, blotted onto nitrocellulose membranes and detected with (A) anti-Rac2 antibody, (B) anti-RhoA antibody, or (C) anti-LyGDI or anti-RhoGDI antibodies. These results were representative for 3 independent experiments with cells from 3 SCN patients and 3 healthy individuals. N indicates normal neutrophils, and SCN, patients' neutrophils.