SDF-1 stimulation enhanced the association of FAK with p130 Cas or paxillin. CTS cells unstimulated or stimulated with SDF-1 for 1 minute were lysed in a lysis buffer and immunoprecipitated with anti-FAK antibody. (A) The immune complexes were resolved on 8% SDS-PAGE and immunoblotted with anti-p130 Cas (upper panel), anti-FAK (lower panel). (B) The immunocomplexes were resolved on 8% SDS-PAGE and immunoblotted with anti-paxillin antibody (upper panel) or anti-FAK antibody (lower panel). TCL represents total cell lysates. The changes in the association of FAK with p130 Cas (A) or with paxillin (B) are indicated (by fold increase) based on the densitometry values.