American Society of Hematology

Fig. 10.

$Fig. 10. Identification of F11 receptor antibodies in the circulation of patients. / The F11 receptor (5 μg applied/lane) was purified from platelet plasma membranes using the affinity chromatography procedures previously described12 and resolved by SDS-PAGE. Immunoblotting was performed using the following antibodies: lane 1, incubation with monoclonal antibody mAb.F11 (4 μg/mL); lane 2, control, incubation with only 2° antibody, rabbit antimouse immunoglobulin; lane 3, incubation with the purified immunoglobulin fraction obtained from the serum of a patient with thrombocytopenia as described in “Materials and methods” (25 μg/mL); lane 4, incubation with plasma from a patient with thrombocytopenia after kidney transplantation, as described in “Materials and methods”; lanes 5 and 6, incubation with plasma obtained from 2 healthy donors and used for immunoblotting; lane 7, control using 2° antibody, rabbit antihuman immunoglobulin.$

Identification of F11 receptor antibodies in the circulation of patients.

The F11 receptor (5 μg applied/lane) was purified from platelet plasma membranes using the affinity chromatography procedures previously described1 2 and resolved by SDS-PAGE. Immunoblotting was performed using the following antibodies: lane 1, incubation with monoclonal antibody mAb.F11 (4 μg/mL); lane 2, control, incubation with only 2° antibody, rabbit antimouse immunoglobulin; lane 3, incubation with the purified immunoglobulin fraction obtained from the serum of a patient with thrombocytopenia as described in “Materials and methods” (25 μg/mL); lane 4, incubation with plasma from a patient with thrombocytopenia after kidney transplantation, as described in “Materials and methods”; lanes 5 and 6, incubation with plasma obtained from 2 healthy donors and used for immunoblotting; lane 7, control using 2° antibody, rabbit antihuman immunoglobulin.

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