Cellular distribution of the F11 receptor as determined by flow cytometry.

Each cell type was incubated with mAb.F11 (5 mg/mL), followed by the addition of the secondary antibody GAM IgG–FITC. Cells were analyzed by FAC Sort flow cytometry. As controls, cells were incubated with nonimmune mouse IgG, followed by incubation with the secondary antibody. Cells considered positive for F11R exhibited a mean fluorescence intensity of 40 channels higher than control groups. Each figure is representative of 5 experiments.