Fig. 7.
Detection of the F11 receptor in human platelets by use of a polyclonal antibody directed against the 21 amino acid sequence of the N-terminus of the purified F11R.
Total platelet proteins, solubilized from approximately 2 × 107 platelets, were resolved on 15% SDS-PAGE. For immunoblotting, an anti-F11R polyclonal antibody, developed against the N-terminal sequence as detailed in “Materials and methods,” was used at a concentration of 20 μg/mL. The arrows point to the 32-kd and 35-kd glycoproteins.
