Fig. 2.
![Fig. 2. Phosphorylation of the F11 receptor after activation of human platelets by thrombin, collagen, and mAb.F11. / Human platelets were incubated with [32P] for 1 hour as described in “Materials and methods” and as previously described.22 Washed platelet suspensions were activated by thrombin, collagen, or mAb.F11 and solubilized by the addition of NP-40 as described.22 Detergent extracts were immunoprecipitated with mAb.F11-coupled Sepharose (A to D) or with uncoupled Sepharose (E to H).22 The autoradiogram demonstrates the immunoprecipitates obtained from platelets treated under the following conditions: A and E, control, nonstimulated; B and F, mAb.F11-stimulated; C and G, thrombin-stimulated; D and H, collagen-stimulated platelets.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/8/10.1182_blood.v95.8.2600/4/bloo00828002w.jpeg?Expires=1725025705&Signature=O-z26aKMFpcsR3oswEolboJXS2dgZG9Dyv0AKb0fcRIeFqBpFKESreTFkejb3SgOuN1g6ChYI41gch6FUHtWNphGEDMozNcid3Czh9obfzEcgRmgF2gt5ZYoT1SrXLpJg9iI0Dp7h5JwxZcs6jJ9IbzcscI5n01jeg33FBp-GBMBDjc-uEcSdeJMnLLdr6w9j~zmT9rlc7U2VDqX1emc2mWocl2sp0HVv2lvns3cj7YkfV1EuF7~qvZhTmc~7NM4F3OI0Q8Lg7wsX09LdkiMxvHS3eFVlBQWdNOGVZ4En1TqjQvWkvfMlPUfLcxoY~jwi0pAvH-Ww~-lK6w999xVmQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Phosphorylation of the F11 receptor after activation of human platelets by thrombin, collagen, and mAb.F11.
Human platelets were incubated with [32P] for 1 hour as described in “Materials and methods” and as previously described.22 Washed platelet suspensions were activated by thrombin, collagen, or mAb.F11 and solubilized by the addition of NP-40 as described.22 Detergent extracts were immunoprecipitated with mAb.F11-coupled Sepharose (A to D) or with uncoupled Sepharose (E to H).22 The autoradiogram demonstrates the immunoprecipitates obtained from platelets treated under the following conditions: A and E, control, nonstimulated; B and F, mAb.F11-stimulated; C and G, thrombin-stimulated; D and H, collagen-stimulated platelets.
