Time-lapse video photographs showing spreading of platelets on monoclonal antibody mAb.F11-coated glass coverslips.

Videos were taken every 5 seconds. Selected frames at 8 different time points are displayed from time 0 (at the instance of platelet application to mAb.F11-coated coverslips to 10.5 minutes later. (A, 0-630 seconds) Demonstrates the spreading of platelets on the mAb.F11-coated surface. (B, 0-630 seconds) Spreading of platelets on the mAb.F11-coated surface in the presence of 300 μmol/L RGDS. (C, 0-630 seconds) Demonstrates the ability of platelets to spread on mAb.F11-coated surfaces in the presence of mAb.IV.3. Note: in the absence of the coated mAb.F11 IgG, the platelets did not adhere onto the surface.